Categories

Organism identification

Organism identification

If organism identification is needed the analysis of DNA fingerprints is method of choice.

For identification so called DNA barcoding method can be used. This method uses a DNA analysis of specific sequences to identify an unknown sample in terms and classify it to particular species.

The most commonly used barcode region for animals is a segment of the mitochondrial gene cytochrome oxidase I (COX), for fungi the more specific sequence is Internal Transcribed Spacer 2 (ITS2) between rRNA genes is used.

For microorganism detection the 16S rRNA gene is the best, because is enough species specific and easily to analyse.

Applications include, for example, products in commerce (herbal supplements, wood, or skins and other animal parts, contamination in protein production, etc.).

Useful links
Recommended products
Dual-labeled hydrolysis probes

Dual-labeled hydrolysis probes Dual-labeled hydrolysis probes

The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.

More
Recommended articles
CRO case studies

Yeast Production Strain Cell Line Authentication by RFLP analysis

Yeast Production Strain Cell Line Authentication by RFLP analysis

Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional

More