Organism identification

If organism identification is needed the analysis of DNA fingerprints is method of choice.

For identification so called DNA barcoding method can be used. This method uses a DNA analysis of specific sequences to identify an unknown sample in terms and classify it to particular species.

The most commonly used barcode region for animals is a segment of the mitochondrial gene cytochrome oxidase I (COX), for fungi the more specific sequence is Internal Transcribed Spacer 2 (ITS2) between rRNA genes is used.

For microorganism detection the 16S rRNA gene is the best, because is enough species specific and easily to analyse.

Applications include, for example, products in commerce (herbal supplements, wood, or skins and other animal parts, contamination in protein production, etc.).

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gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Alternative dyes for oligonucleotides/probes labeling

Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.