Contamination by production organism residual DNA
Plasmid DNA is frequently used in gene therapy and high purity plasmids are required. Residual DNA or proteins from the production organism (where plasmid was produced e.g. E. coli) are considered unacceptable contaminant.
Contamination by wild type virus in artificial drug virus
Viral vaccines or vectors are mostly prepared from wild type precursors, which lost their original activity. Artificial virus is less dangerous than wild type and has special characteristics. Wild type virus in a vaccine could have dangerous effect.
Viral contaminants in virus-free material
For the control of biological material it is necessary to check if the material does not contain any hazardous biological substance, such as virus.
Contamination tests on request development of method and subsequent validation can be included
gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.