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Gene quantification (qPCR)

Gene quantification (qPCR)

  • Contamination by production organism residual DNA
    Plasmid DNA is frequently used in gene therapy and high purity plasmids are required. Residual DNA or proteins from the production organism (where plasmid was produced e.g. E. coli) are considered unacceptable contaminant.
  • Contamination by wild type virus in artificial drug virus
    Viral vaccines or vectors are mostly prepared from wild type precursors, which lost their original activity. Artificial virus is less dangerous than wild type and has special characteristics. Wild type virus in a vaccine could have dangerous effect.
  • Viral contaminants in virus-free material
    For the control of biological material it is necessary to check if the material does not contain any hazardous biological substance, such as virus.
  • Contamination tests on request
    development of method and subsequent validation can be included

Quality Control Quality control

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Dual-labeled hydrolysis probes

Dual-labeled hydrolysis probes Dual-labeled hydrolysis probes

The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.

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Yeast Production Strain Cell Line Authentication by RFLP analysis

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Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional

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