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Gene quantification (qPCR)

Gene quantification (qPCR)

  • Contamination by production organism residual DNA
    Plasmid DNA is frequently used in gene therapy and high purity plasmids are required. Residual DNA or proteins from the production organism (where plasmid was produced e.g. E. coli) are considered unacceptable contaminant.
  • Contamination by wild type virus in artificial drug virus
    Viral vaccines or vectors are mostly prepared from wild type precursors, which lost their original activity. Artificial virus is less dangerous than wild type and has special characteristics. Wild type virus in a vaccine could have dangerous effect.
  • Viral contaminants in virus-free material
    For the control of biological material it is necessary to check if the material does not contain any hazardous biological substance, such as virus.
  • Contamination tests on request
    development of method and subsequent validation can be included

Quality Control Quality control

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    Recommended products
    gb Easy PCR Master Mix

    gb Easy PCR Master Mix gb Easy PCR Master Mix

    gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.

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    Yeast Production Strain Cell Line Authentication by RFLP analysis

    Yeast Production Strain Cell Line Authentication by RFLP analysis

    Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional

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