Info Array 1

Array I layout
plate 1 2 3 4 5 6 7 8 9 10 11 12
A DNMT3B PODXL ZFP42 ACTC1 BRIX1 CD9 CDH1 FGF4 FOXD3 GABRB3 GRB7 LEFTY1
B NODAL REST RUNX2 TERT ALDH1A1 ALDH2 APC CDH2 FGFR1 HDAC2 KAT2A KAT7
C KAT8 PARD6A COMMD3 IL6ST LIFR SFRP2 OCT4 SOX2 KLF4 LIN28A NANOG NR5A2
D UTF1 ALPL GDF3 TDGF1 CCNA2 CDK1 CDC42 DPPA2 DPPA3 FGF2 HSPA9 MYBL2
E OTX2 SOX15 TBX3 TCF3 CD34 GJB2 KRT15 COL1A1 COL2A1 EMX2 FABP7 NCAM1
F NES NUMB OLIG2 PECAM1 SYCP3 PAX6 TUBB3 FGF8 FN1 GFAP NEUROD1 TH
G BMP4 GATA2 HAND1 MESP1 HBB RUNX1 FOXA2 GATA4 HHEX HNF4A SOX17 EOMES
H KLF2 TFCP2L1 DNMT1 DNMT3A DNMT3L XIST GAPDH B2M SDHA HPRT1 NO-RT control PCR positive control

 

Cartilage Neuronal Stem Cell Cardiomyocyte Germ cells Other Reprogramming Factors Mesoderm Endoderm Naive gene markers House keeping genes
iPSC Markers Other iPSC-Related Genes Reprogramming Factors Ectoderm Reprogramming Enhancers & Inhibitors ESC Markers Other ESC-Related Genes Keratinocyte Blood Stem Cell

 

list of genes

Function # Array position
Gene Symbol Gene Name Description GeneBank
iPSC Markers 1 A1 DNMT3B ICF, ICF1, M.HsaIIIB DNA (cytosine-5-)-methyltransferase 3 beta NM_006892
2 A2 PODXL Gp200, PC, PCLP, PCLP-1 Podocalyxin-like NM_005397
3 A3 ZFP42 REX1, ZNF754 Zinc finger protein 42 homolog (mouse) NM_174900
Other iPSC-Related Genes 4 A4 ACTC1 ACTC, ASD5, CMD1R, CMH11, LVNC4 Actin, alpha, cardiac muscle 1 NM_005159
5 A5 BRIX1 (BXDC2) BRIX, BXDC2 BRX1, biogenesis of ribosomes, homolog (S. cerevisiae) NM_018321
6 A6 CD9 BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29 CD9 molecule NM_001769
7 A7 CDH1 Arc-1, CD324, CDHE, ECAD, LCAM, UVO Cadherin 1, type 1, E-cadherin (epithelial) NM_004360
8 A8 FGF4 HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF Fibroblast growth factor 4 NM_002007
9 A9 FOXD3 AIS1, Genesis, HFH2, VAMAS2 Forkhead box D3 NM_012183
10 A10 GABRB3 ECA5 Gamma-aminobutyric acid (GABA) A receptor, beta 3 NM_000814
11 A11 GRB7 Growth factor receptor-bound protein 7 NM_005310
12 A12 LEFTY1 LEFTB, LEFTYB Left-right determination factor 1 NM_020997
13 B1 NODAL HTX5 Nodal homolog (mouse) NM_018055
14 B2 REST NRSF, XBR RE1-silencing transcription factor NM_005612
15 B3 RUNX2 AML3, CBF-alpha-1, CBFA1, CCD, CCD1, CLCD, OSF-2, OSF2, PEA2aA, PEBP2aA Runt-related transcription factor 2 NM_004348
16 B4 TERT CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT Telomerase reverse transcriptase NM_198253
17 B5 ALDH1A1 ALDC, ALDH-E1, ALDH1, ALDH11, HEL-S-53e, HEL12, PUMB1, RALDH1 Aldehyde dehydrogenase 1 family, member A1 NM_000689
18 B6 ALDH2 ALDH-E2, ALDHI, ALDM Aldehyde dehydrogenase 2 family (mitochondrial) NM_000690
19 B7 APC BTPS2, DP2, DP2.5, DP3, GS, PPP1R46 Adenomatous polyposis coli NM_000038
20 B8 CDH2 CD325, CDHN, CDw325, NCAD Cadherin 2, type 1, N-cadherin (neuronal) NM_001792
21 B9 FGFR1 BFGFR, CD331, CEK, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1 Fibroblast growth factor receptor 1 NM_015850
22 B10 HDAC2 HD2, RPD3, YAF1 Histone deacetylase 2 NM_001527
23 B11 KAT2A GCN5, GCN5L2, PCAF-b, hGCN5 K(lysine) acetyltransferase 2A NM_021078
24 B12 KAT7 HBO1, HBOA, MYST2, ZC2HC7 K(lysine) acetyltransferase 7 NM_007067
25 C1 KAT8 MOF, MYST1, ZC2HC8, hMOF K(lysine) acetyltransferase 8 NM_032188
26 C2 PARD6A PAR-6A, PAR6, PAR6C, PAR6alpha, TAX40, TIP-40 Par-6 partitioning defective 6 homolog alpha (C. elegans) NM_016948
27 C3 COMMD3 BUP, C10orf8, RP11-573G6.3 COMM domain containing 3 NM_012071
28 C4 IL6ST CD130, CDW130, GP130, IL-6RB interleukin 6 signal transducer (gp130, oncostatin M receptor) NM_175767
29 C5 LIFR CD118, LIF-R, SJS2, STWS, SWS leukemia inhibitory factor receptor alpha NM_002310
30 C6 SFRP2 FKSG12, FRP-2, SARP1, SDF-5 secreted frizzled-related protein 2 NM_003013
Reprogramming Factors 31 C7 POU5F1 (Oct3/4) OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 POU class 5 homeobox 1 NM_002701
32 C8 SOX2 ANOP3, MCOPS3 SRY (sex determining region Y)-box 2 NM_003106
33 C9 KLF4 EZF, GKLF Kruppel-like factor 4 (gut) NM_004235
Other Reprogramming Factors: 34 C10 LIN28A CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A Lin-28 homolog A (C. elegans) NM_024674
35 C11 NANOG Nanog homeobox NM_024865
36 C12 NR5A2 B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2 Nuclear receptor subfamily 5, group A, member 2 NM_003822
Reprogramming Enhancers & Inhibitors 37 D1 UTF1 Undifferentiated embryonic cell transcription factor 1 NM_003577
ESC Markers 38 D2 ALPL AP-TNAP, APTNAP, HOPS, TNAP, TNSALP Alkaline phosphatase, liver/bone/kidney NM_000478
39 D3 GDF3 KFS3, MCOP7, MCOPCB6 Growth differentiation factor 3 NM_020634
40 D4 TDGF1 (CRIPTO) CR, CRGF, CRIPTO Teratocarcinoma-derived growth factor 1 NM_003212
Other ESC-Related Genes: . 41 D5 CCNA2 CCN1, CCNA Cyclin A2 NM_001237
42 D6 CDK1 (CDC2) CDC2, CDC28A, P34CDC2 Cyclin-dependent kinase 1 NM_001786
43 D7 CDC42 CDC42Hs, G25K Cell division cycle 42 (GTP binding protein, 25kDa) NM_001791
44 D8 DPPA2 CT100, ECAT15-2, PESCRG1 Developmental pluripotency associated 2 NM_138815
45 D9 DPPA3 STELLA Developmental pluripotency associated 3 NM_199286
46 D10 FGF2 BFGF, FGF-2, FGFB, HBGF-2 Fibroblast growth factor 2 (basic) NM_002006
47 D11 HSPA9 CSA, GRP-75, GRP75, HSPA9B, MOT, MOT2, MTHSP75, PBP74 Heat shock 70kDa protein 9 (mortalin) NM_004134
48 D12 MYBL2 B-MYB, BMYB V-myb myeloblastosis viral oncogene homolog (avian)-like 2 NM_002466
49 E1 OTX2 CPHD6, MCOPS5 Orthodenticle homeobox 2 NM_021728
50 E2 SOX15 SOX20, SOX26, SOX27 SRY (sex determining region Y)-box 15 NM_006942
51 E3 TBX3 TBX3-ISO, UMS, XHL T-box 3 NM_016569
52 E4 TCF3 E2A, E47, ITF1, TCF-3, VDIR, bHLHb21 Transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) NM_003200
Blood Stem Cell: 53 E5 CD34 CD34 molecule NM_001773
Keratinocyte: 54 E6 GJB2 CX26, DFNA3, DFNA3A, DFNB1, DFNB1A, HID, KID, NSRD1, PPK Gap junction protein, beta 2, 26kDa NM_004004
55 E7 KRT15 CK15, K15, K1CO Keratin 15 NM_002275
Cartilage: 56 E8 COL1A1 OI4 Collagen, type I, alpha 1 NM_000088
57 E9 COL2A1 ANFH, AOM, COL11A3, SEDC, STL1 Collagen, type II, alpha 1 NM_001844
Neuronal Stem Cell: 58 E10 EMX2 Empty spiracles homeobox 2 NM_004098
59 E11 FABP7 B-FABP, BLBP, FABPB, MRG Fatty acid binding protein 7, brain NM_001446
60 E12 NCAM1 CD56, MSK39, NCAM Neural cell adhesion molecule 1 NM_000615
61 F1 NES Nestin NM_006617
62 F2 NUMB C14orf41, S171, c14_5527 Numb homolog (Drosophila) NM_003744
63 F3 OLIG2 BHLHB1, OLIGO2, PRKCBP2, RACK17, bHLHe19 Oligodendrocyte lineage transcription factor 2 NM_005806
Cardiomyocyte 64 F4 PECAM1 CD31, CD31, EndoCAM, GPIIA‘, PECA1, PECAM-1, endoCAM Platelet/endothelial cell adhesion molecule NM_000442
Germ Cells 65 F5 SYCP3 COR1, SCP3, SPGF4 Synaptonemal complex protein 3 NM_153694
Ectoderm: 66 F6 PAX6 AN, AN2, D11S812E, MGDA, WAGR Paired box 6 NM_000280
67 F7 TUBB3 CDCBM, CDCBM1, CFEOM3A, TUBB4, beta-4 Tubulin, beta 3 NM_006086
68 F8 FGF8 AIGF, FGF-8, HBGF-8, HH6, KAL6 fibroblast growth factor 8 (androgen-induced) NM_006119
69 F9 FN1 CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF fibronectin 1 NM_212482.2
70 F10 GFAP glial fibrillary acidic protein NM_002055
71 F11 NEUROD1 BETA2, BHF-1, MODY6, NEUROD, bHLHa3 neuronal differentiation 1 NM_002500
72 F12 TH DYT14, DYT5b, TYH tyrosine hydroxylase NM_199293
Mesoderm: 73 G1 BMP4 BMP2B, BMP2B1, MCOPS6, OFC11, ZYME Bone morphogenetic protein 4 NM_001202
74 G2 GATA2 DCML, MONOMAC, NFE1B GATA binding protein 2 NM_032638
75 G3 HAND1 Hxt, Thing1, bHLHa27, eHand Heart and neural crest derivatives expressed 1 NM_004821
76 G4 MESP1 bHLHc5 Mesoderm posterior 1 homolog (mouse) NM_018670
77 G5 HBB CD113t-C, beta-globin hemoglobin, beta NM_000518
78 G6 RUNX1 AML1, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2aB Runt-related transcription factor 1 NM_001754
Endoderm: 79 G7 FOXA2 HNF3B, TCF3B Forkhead box A2 NM_021784
80 G8 GATA4 ASD2, TACHD, VSD1 GATA binding protein 4 NM_002052
81 G9 HHEX HEX, HMPH, HOX11L-PEN, PRH, PRHX Hematopoietically Expressed Homeobox NM_002729
82 G10 HNF4A HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14 Hepatocyte nuclear factor 4, alpha NM_178849
83 G11 SOX17 VUR3 SRY (sex determining region Y)-box 17 NM_022454
84 G12 EOMES TBR2 eomesodermin NM_005442.3
Naive gene markers 85 H1 KLF2 LKLF Kruppel-like factor 2 (lung) NM_016270.3
86 H2 TFCP2L1 CRTR1, LBP-9, LBP9 Homo sapiens transcription factor CP2-like 1 (TFCP2L1) NM_014553.2
87 H3 DNMT1 ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT DNA (cytosine-5-)-methyltransferase 1 NM_001130823.2
NM_001379.3
88 H4 DNMT3A DNMT3A2, M.HsaIIIA DNA (cytosine-5-)-methyltransferase 3 alpha NM_175629.2
NM_153759.3
NM_022552.4
89 H5 DNMT3L DNA (cytosine-5-)-methyltransferase 3-like NM_013369.3
NM_175867.2
90 H6 XIST DXS1089, DXS399E, LINC00001, NCRNA00001, SXI1, swd66 X inactive specific transcript (non-protein coding) NR_001564.2
House keeping genes 91 H7 GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase NM_002046.6
92 H8 B2M IMD43 beta-2-microglobulin NM_004048.3
93 H9 SDHA CMD1GG, FP, PGL5, SDH1, SDH2, SDHF succinate dehydrogenase complex flavoprotein subunit A NM_004168.3
94 H10 HPRT1 HGPRT, HPRT hypoxanthine phosphoribosyltransferase 1 NM_000194.2
NO-RT control (primery GAPDH) 95 H11 NO-RT control
PCR positive control (primery ACTB) 96 H12 PCR positive control