Categories

DNA/RNA integrity

DNA/RNA integrity

Integrity of DNA/RNA is very important test needed according to the Europe pharmacopeia chapter 5.14.

Mostly the test can be done as agarose gel electrophoresis or using advanced techniques such as PCR or fragment analysis.

All the tests are according to the Europe pharmacopeia test 2.6.21.

To this test belong also plasmid DNA conformation test, because  Stability of plasmid DNA depends on its conformation and the right conformation  is required (e.g. supercoiled form).

For RNA integrity determination of RIN is important as well.

Useful links
Recommended products
Dual-labeled hydrolysis probes

Dual-labeled hydrolysis probes Dual-labeled hydrolysis probes

The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.

More
Recommended articles
CRO case studies

Yeast Production Strain Cell Line Authentication by RFLP analysis

Yeast Production Strain Cell Line Authentication by RFLP analysis

Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional

More