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Plasmid linearization

If the plasmid DNA is intended for use as a PCR template, it is recommended to use it as a linear DNA. A circular plasmid mostly has a supercoiled conformation, where the target sequence is less accessible for primers and for polymerase.

This service follows plasmid amplification and isolation. Linear DNA provides more reproducible and accurate results. Linearization is performed using restriction endonucleases. The cleaved product is further purified and quantified.

Product parameters

isolate of a linearized plasmid in the minimal amount of 10 μg
concentration min 1E¹⁰ copies/μl
volume of 200 μl

The cleaved product is further purified and quantified.

Output

Finalized plasmid is delivered dissolved in sterile TE buffer (or in sterile deionized water on request). We provide you with information about plasmid concentration and purity.

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Cat. No.	Product	No. of reactions	Price

1850

Plasmid linearization

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Shiping on dry ice from € 110 (EU)
For other destinations see indicative list.

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Useful links

Dual-labeled hydrolysis probes

The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.

Alternative dyes for oligonucleotides/probes labeling

Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.