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Patented dye alternatives

Oligonucleotides and probes labeled with fluorescent dyes are commonly used in a variety of molecular biology methods. Some of the dyes may be patented, often lowering the number of available suppliers and usually also affecting affordability of such oligonucleotides.

However, once a few necessary considerations are made (see the article in the link below), most of the patented dyes can be effectively replaced with more available nad cost-effective alternatives. The table below shows a list of most commonly used patented dyes with corresponding suggested alternatives, all of which can be synthesised in GENERI BIOTECH.

Why choose alternative dyes instead of “genuine” dyes?

Patented dye Abs/Em Common alternative Abs/Em ATTO alternative* Abs/Em
VIC 538/554 HEX 537/556 ATTO 532 532/552
CAL Fluor Orange 560 538/559
Yakima Yellow 530/549
NED 553/575 Cy 3 547/563 ATTO 550 554/576
Pet 558/595 ROX 575/602 ATTO 565 564/590
Cy 3.5 591/604
CAL Fluor Red 610 590/610
LIZ 638/655 ATTO 633 630/651
Alexa Fluor 488 495/519 FAM 495/520 ATTO 488 500/520
Alexa Fluor 514 517/542 JOE 529/555 ATTO 514 511/532
TET 519/539
Alexa Fluor 532 532/554 JOE 529/555 ATTO 532 532/552
HEX 537/556
Alexa Fluor 555 555/565 Cy 3 547/563 ATTO 550 554/576
Alexa Fluor 546 556/573 Cy 3 547/563 ATTO 550 554/576
Alexa Fluor 594 590/617 ROX 575/602 ATTO 590 593/622
Alexa Fluor 647 650/665 Cy 5 646/662 ATTO 647N 646/664
Alexa Fluor 660 663/690 Cy 5.5 672/692 ATTO 680 681/698
ATTO 700 700/716
Alexa Fluor 680 679/702 Cy 5.5 672/692 ATTO 680 681/698
ATTO 700 700/716
LC Red 610 590/610 ROX 575/602 ATTO 610 616/633
CAL Fluor Red 610 590/610
LC Red 670 650/670 Cy 5 646/662 ATTO 647N 646/664
LC Red 640 625/640 ROX 575/602 ATTO 620 620/642
CAL Fluor Red 635 618/637
LC Red 705 680/710 Cy 5.5 672/692 ATTO 680 681/698
ATTO 700 700/716

ATTO dyes are covered by patents as well, however GENERI BIOTECH is licenced to use them for oligo/probe custom synthesis.