Oligonucleotides and probes labeled with fluorescent dyes are commonly used in a variety of molecular biology methods. Some of the dyes may be patented, often lowering the number of available suppliers and usually also affecting affordability of such oligonucleotides.
However, once a few necessary considerations are made (see the article in the link below), most of the patented dyes can be effectively replaced with more available nad cost-effective alternatives. The table below shows a list of most commonly used patented dyes with corresponding suggested alternatives, all of which can be synthesised in GENERI BIOTECH.
Why choose alternative dyes instead of “genuine” dyes?
Patented dye | Abs/Em | Common alternative | Abs/Em | ATTO alternative* | Abs/Em |
---|---|---|---|---|---|
VIC | 538/554 | HEX | 537/556 | ATTO 532 | 532/552 |
CAL Fluor Orange 560 | 538/559 | ||||
Yakima Yellow | 530/549 | ||||
NED | 553/575 | Cy 3 | 547/563 | ATTO 550 | 554/576 |
Pet | 558/595 | ROX | 575/602 | ATTO 565 | 564/590 |
Cy 3.5 | 591/604 | ||||
CAL Fluor Red 610 | 590/610 | ||||
LIZ | 638/655 | ATTO 633 | 630/651 | ||
Alexa Fluor 488 | 495/519 | FAM | 495/520 | ATTO 488 | 500/520 |
Alexa Fluor 514 | 517/542 | JOE | 529/555 | ATTO 514 | 511/532 |
TET | 519/539 | ||||
Alexa Fluor 532 | 532/554 | JOE | 529/555 | ATTO 532 | 532/552 |
HEX | 537/556 | ||||
Alexa Fluor 555 | 555/565 | Cy 3 | 547/563 | ATTO 550 | 554/576 |
Alexa Fluor 546 | 556/573 | Cy 3 | 547/563 | ATTO 550 | 554/576 |
Alexa Fluor 594 | 590/617 | ROX | 575/602 | ATTO 590 | 593/622 |
Alexa Fluor 647 | 650/665 | Cy 5 | 646/662 | ATTO 647N | 646/664 |
Alexa Fluor 660 | 663/690 | Cy 5.5 | 672/692 | ATTO 680 | 681/698 |
ATTO 700 | 700/716 | ||||
Alexa Fluor 680 | 679/702 | Cy 5.5 | 672/692 | ATTO 680 | 681/698 |
ATTO 700 | 700/716 | ||||
LC Red 610 | 590/610 | ROX | 575/602 | ATTO 610 | 616/633 |
CAL Fluor Red 610 | 590/610 | ||||
LC Red 670 | 650/670 | Cy 5 | 646/662 | ATTO 647N | 646/664 |
LC Red 640 | 625/640 | ROX | 575/602 | ATTO 620 | 620/642 |
CAL Fluor Red 635 | 618/637 | ||||
LC Red 705 | 680/710 | Cy 5.5 | 672/692 | ATTO 680 | 681/698 |
ATTO 700 | 700/716 |
ATTO dyes are covered by patents as well, however GENERI BIOTECH is licenced to use them for oligo/probe custom synthesis.