Modification by group: Fluorophores
Position of modification: 5´
Tetrachlorofluorescein is commonly used for multiplex reactions with FAM and HEX. It can be linked to an oligonucleotide at the 5 ‘end.
Absorption maximum: 519 nm
Emission maximum: 539 nm
| Cat. No. | Modification | Scale | Price |
|---|
| 1114-040 | 5´- TET | 40 nmol |
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| 1114-200 | 5´- TET | 200 nmol |
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Oligo price = price of sequence without modification + surcharge for modification
Shiping at ambient temperature from
€ 40 (EU)
For other destinations see
indicative list.
General Terms and Conditions
Dual-labeled hydrolysis probes The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Minimum 3 weeks
Shipping
At room temperature
Storage recommendation
Dried oligonucleotides: at 4 °C (in a refrigerator)
Dissolved: -20°C (in a freezer), we recommend to store in aliquotes
Product expiry date
24 months form the date of manufacture (provided the product is stored as recommended)
The provided information is only indicative and is always subject to the specific content and extent of an order.
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