Modification by group: Fluorophores

Position of modification:

6-karboxy-4′, 5′-dichlor-2′, 7′-dimethoxyfluorescein is commonly used in multiplex reactions together with FAM. JOE may be attached to the 5′ end of the oligonucleotide.

Absorption maximum: 529 nm

Emission maximum: 555 nm

Cat. No. Modification Scale Price
1122-5 5' - JOE 40 nmol
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1122-5 5' - JOE 200 nmol
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Modification is limited and can be performed upon consultation; precise price quotation can be prepared after providing particular sequences.

Oligo price = price of sequence without modification + surcharge for modification

Shiping at ambient temperature from € 40 (EU)
For other destinations see indicative list.

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Dual-labeled hydrolysis probes

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The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.

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