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Fluorescein

Modification by group: Fluorophores

Position of modification: 3´, 5´

It is a mixture of 5- and 6-carboxyfluorescein isomers. Fluorescein may be attached anywhere within the oligonucleotide sequence.

Absorption maximum: 494 nm

Emission maximum: 522 nm

Cat. No.	Modification	Scale	Price

1109-040

5´- Fluorescein

40 nmol

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1109-200

5´- Fluorescein

200 nmol

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1109-001

5´- Fluorescein

1 µmol

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Oligo price = price of sequence without modification + surcharge for modification

3´- Fluorescein

Cat. No.	Modification	Scale	Price

1133-200

3´- Fluorescein

200 nmol

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Shiping at ambient temperature from € 45 (EU)
For other destinations see indicative list.

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gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Alternative dyes for oligonucleotides/probes labeling

Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.