Modification by group: Modified backbone
Position of modification: 3´, 5´, internal
Phosphorothioates contain the exchange of one oxygen in the phosphate bond with sulfur. By exchanging oxygen for sulfur, the resistance of the oligonucleotide to nucleases increases.
pure phosphorothioates: substitution is made in all phosphate linkages in the chain
mixed phosphorothioates: substitution is only made on selected phosphate bonds.
Phosphorothioates are used for antisense applications where a 10 μmol scale is usually selected.
| Cat. No. | Modification | Scale | Price |
|---|
| 2104-040 | Phosphorothioate, 3´end | 40 nmol |
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| 2104-200 | Phosphorothioate, 3´end | 200 nmol |
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| 2104-001 | Phosphorothioate, 3´end | 1 µmol |
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Oligo price = price of sequence without modification + surcharge for modification
| Cat. No. | Modification | Scale | Price |
|---|
| 1160-020 | Phosphorothioate, internal | 20 nmol |
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| 1160-040 | Phosphorothioate, internal | 40 nmol |
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| 1160-200 | Phosphorothioate, internal | 200 nmol |
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| 1160-001 | Phosphorothioate, internal | 1 µmol |
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Oligo price = price of sequence without modification + surcharge for modification
| Cat. No. | Modification | Scale | Price |
|---|
| 1453-040 | Phosphorothioate, 5´end | 40 nmol |
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| 1453-200 | Phosphorothioate, 5´end | 200 nmol |
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| 1453-001 | Phosphorothioate, 5´end | 1 µmol |
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Oligo price = price of sequence without modification + surcharge for modification
Shiping at ambient temperature from
€ 45 (EU)
For other destinations see
indicative list.
General Terms and Conditions
gb OneStep RT-qPCR Kit gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Term B: products are dispatched within the next 5 full business days after receiving the order
Shipping
At room temperature
Storage recommendation
Dried oligonucleotides: at 4 °C (in a refrigerator)
Dissolved: -20°C (in a freezer), we recommend to store in aliquotes
Product expiry date
24 months form the date of manufacture (provided the product is stored as recommended)
The provided information is only indicative and is always subject to the specific content and extent of an order.
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