Modification by group: ATTO Fluorophores
ATTO dyes are a series of fluorescent dyes which provide all the crucial properties required for modern fluorescent technologies with spectral region from 350 nm in the UV to 750 nm in the near infrared.
ATTO-dyes represent a new generation of patented fluorescent labels developed directly for biomolecular labels in the field of life sciences.
The common advantages of these colors are: high absorption, high fluorescence emission and high photostability.
ATTO dyes parameters: absorption and emissions wavelengths, replacing dyes
ATTO dyes cover a wide spectrum range from 350 nm to 750 nm. Due to this range of absorption and emission spectrums, these tags can be a convenient alternative to all commonly used fluorophores (see the table).
ATTO also offers fluorescents that exhibit an extremely wide Stokes shift, which makes them very suitable for use in multiplexing.
Labeling of the oligonucleotides with ATTO dyes takes place post-synthetically, so it is necessary to attach an amino group to the oligonucleotide at the site of the desired binding, through which the self-binding of the ATTO dye is in the form of the NHS ester.
After labeling the oligonucleotide with ATTO dyes, HPLC purification is recommended to remove dye which is used in excess for labeling.
General Terms and Conditions
Custom oligo synthesis In GENERI BIOTECH oligonucleotides are synthesized on Highly cross-linked polystyrene beads supports. Oligonucleotides thus synthesized have a very low representation of truncated shorter chains compared to normal CPG supports.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Minimum 3 weeks
Shipping
At room temperature
Storage recommendation
Dried oligonucleotides: at 4 °C (in a refrigerator)
Dissolved: -20°C (in a freezer), we recommend to store in aliquotes
Product expiry date
24 months form the date of manufacture (provided the product is stored as recommended)
The provided information is only indicative and is always subject to the specific content and extent of an order.
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