Detection method: allelic discrimination, real-time PCR

The kit is intended for detection of mutation V34L in fibrin stabilizing factor XIII in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).

Clinical implications

Factor XIII, also called fibrin stabilizing factor, is a proenzym circulating in plasma. Its active form stabilizes the fibrin network by catalytic formation of covalent bonds between fibrin molecules and other proteins (alpha-2-antiplasmin, fibronectin, collagen), thereby protecting the coagulum against fibrinolysis.

FXIIIa plays an important role not only in hemostasis but also during wound healing and in maintaining pregnancy. The presence of the V34L has a protective effect against myocardial infarction and venous thrombosis. On the other hand, it is also stated that the presence of this polymorphism is associated with an increased risk of recurrent pregnancy loss in the first trimester, which was also found in connection with the PAI-1 (4G/5G) mutation.

Parameters of the diagnostic kit

  • ready-to-use assay
  • sample concentration 1-100 ng/µl
  • positive and negative controls included
  • FAM and HEX channels detection
  • identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
Cat. No. Product No. of reactions Price
3209-025 gb HEMO FXIII (V34L) 25
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3209 gb HEMO FXIII (V34L) 100
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1 kit contains reagents to provide 25 or 100 PCR reactions (20 μl volume of each reaction).

Validated for:

ABI 7500/7500 Fast (ABI)

ABI 7900HT (ABI)

AriaMx (Agilent Technologies)

CFX96/96Touch (Bio-Rad)

iCycler iQ5 (Bio-Rad)


RG 3000 (Corbett Research)

RG 6000/Q (Corbett Research/Qiagen)

SmartCycler (Cepheid)

Select further cyclers

Useful links
Recommended products
gb SG PCR Master Mix

gb SG PCR Master Mix gb SG PCR Master Mix

gb SG PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

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