gb HEMO GPIa (C807T)

gb HEMO GPIa (C807T)

Detection method: allelic discrimination, real-time PCR

The kit is intended for detection of C807T mutation in GPIa gene in human genomic DNA. Detection is based on realtime polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).

Clinical implications

Platelet glycoprotein GPIa forms a complex GPIa/IIa with glycoprotein GPIIa on the surface of blood platelets. One-nucleotide C807T mutation is one of the “silent” polymorphisms that do not change the amino acid sequence. However, the presence of the mutated form increases the gene expression.

Both, heterozygous and homozygous carriers of the mutated allele, have twice as many receptors on the platelet surface as compared to the wild type homozygotes, which causes an increased thrombocyte adhesivity to type 1 collagen. Under high shear stress in the arterial bed, increased thrombocyte adhesivity has thrombogenic potential. Both, heterozygous and homozygous carriage of the mutated allele, is considered to be a mild risk factor of myocardial infarction and stroke in younger people.

Parameters of the diagnostic kit

  • ready-to-use assay
  • sample concentration 1-100 ng/µl
  • positive and negative controls included
  • FAM and HEX channels detection
  • identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
Cat. No. Product No. of reactions Price
3211-025 gb HEMO GPIa (C807T) 25
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3211 gb HEMO GPIa (C807T) 100
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1 kit contains reagents to provide 25 or 100 PCR reactions (20 μl volume of each reaction).

Validated for:

ABI 7300 (ABI)

ABI 7500/7500 Fast (ABI)

AriaMx (Agilent Technologies)

CFX96/96Touch (Bio-Rad)

iCycler iQ5 (Bio-Rad)

MIC (BMS)

RG 3000 (Corbett Research)

RG 6000/Q (Corbett Research/Qiagen)

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