This service is recommended when it is necessary to verify whether a correct sequence was cloned in, whether plasmids were not misidentified (mistaken by accident), or to verify that there are no errors in the plasmid sequence.
The following methods are most commonly used to verify accuracy of a plasmid:
1) Restriction analysis enabling the recognition of more extensive deletions (typically if a target sequence was not cloned in) or an accidental interchange of plasmids (typically if plasmids do not originate from an original source).
2) Plasmid conformation determination suitable for quality check of a native plasmid. When native, a plasmid should be present prevailingly in supercoiled conformation. In order to verify the conformation, plasmid analysis on gel is used and a percentage of the supercoiled form is determined.
Plasmid verification can also be performed under GMP or GLP regulations.
We provide the customer with a reporting protocol. If the customer determines acceptability criteria, it is possible to issue a report stating that the plasmid complies with the determined parameters.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 1855-R | Plasmid verification by restriction analysis |
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| 1855-C | Plasmid verification by conformation determination |
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Shiping at ambient temperature from
€ 40 (EU)
For other destinations see
indicative list.
General Terms and Conditions
gb OneStep RT-qPCR Kit gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Test output is in the form of a reporting protocol. If the customer provides so-called acceptability criteria, it is possible to issue a report stating that plasmid meets/does not meet these criteria.
Dispatch date
According to requirements on the method for plasmid verification
Shipping
Reporting protocol in a printed version – by Czech Post or electronically by email
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