Plasmid verification

This service is recommended when it is necessary to verify whether a correct sequence was cloned in, whether plasmids were not misidentified (mistaken by accident), or to verify that there are no errors in the plasmid sequence.

Description of the service

The following methods are most commonly used to verify accuracy of a plasmid:

1) Restriction analysis enabling the recognition of more extensive deletions (typically if a target sequence was not cloned
in) or an accidental interchange of plasmids (typically if plasmids do not originate from an original source).

2) Sequencing verifying the sequence of a plasmid and detecting even single-point mutations.

3) Plasmid conformation determination suitable for quality check of a native plasmid. When native, a plasmid should be
present prevailingly in supercoiled conformation. In order to verify the conformation, a plasmid analysis on gel is used
and a percentage of the supercoiled form is determined.

Plasmid verification can also be performed under GMP or GLP regulations.

Output of the test

We provide the customer with a reporting protocol. If the customer determines acceptability criteria, it is possible to issue a report stating that the plasmid complies with the determined parameters.

Cat. No. Product No. of reactions Price
1855-R Plasmid verification by restriction analysis
Add to Quote
1855-S Plasmid verification by sequencing
Add to Quote
1855-C Plasmid verification by conformation determination
Add to Quote
Useful links
Recommended products
Kit for isolation of DNA from tissues

Kit for isolation of DNA from tissues Kit for isolation of DNA from tissues

The kit enables you to achieve highly pure genomic DNA from various types of human or animal tissues and cultured cells, buccal swabs or paraffin-embedded tissue. Moreover, microbial, fungal or yeast DNA can be efficiently isolated as well. High DNA yields, reproducible results and fast working method are guaranteed.

More
Recommended articles