Detection method: allelic discrimination
The CE IVD kit is used for determination of genotype of five polymorphisms: A (D70G, rs1799807), K (A539T, rs1803274), F1 (T243M, rs28933389), F2 (G390V, rs28933390), S1 (FS117, rs398124632) in human genomic DNA. The principle of detection is based on real-time PCR by fluorescently labelled probes (allelic discrimination).
Butyrylcholinesterase (BCHE) is an enzyme involved in the metabolism of the myorelaxans suxamethonium (succinylcholine) and mivacurium. Decreased activity of this enzyme results in higher plasma drug concentrations and prolonged muscle paralysis associated with the need for pulmonary ventilation. The most common genetic predispositions leading to pseudocholinesterase deficiency include the BCHE variants A, K, F and S1.
In patients diagnosed with genetic alterations in the BCHE gene, it is recommended to use alternative agents to myorelaxants suxamthonium and mivacurium, which have available antidotes, eliminating the risk to the patient and the financial cost of subsequent artificial pulmonary ventilation.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3256-025 | gb PHARM BCHE | 25 |
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1 CE IVD kit contains reagents to provide 25 PCR reactions (20 μl volume of each reaction).
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General Terms and Conditions
Validated for:
CFX Opus 96 (Bio-Rad)
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
Dual-labeled hydrolysis probes The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Products are dispatched within 10 business days upon receiving the order
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Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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