gb Ideal PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
gb PHARM UGT1A1
Detection method: melting analysis
This in vitro diagnostic kit allows detection of variants of 5TA (UGT1A1*36), 6TA (UGT1A1*1), 7TA (UGT1A1*28) and 8TA (UGT1A1*37) polymorphisms. The detection is based on the Q-FRET probe and uses the analysis of melting curves for multiplex genotyping.
Clinical implications of the CE IVD kit
The UGT1A1 gene is a part of a complex locus that encodes several UDP-glucuronosyltransferases, enzymes of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. Mutations in the UGT1A1 gene lead to mulfunctions in conjugation phase of bilirubin biotransformation.
The gb PHARM UGT1A1 diagnostic kit is intended to determine changes in a number of TA-repetitions in a UGT1A1 gene promoter (rs8175347, UGT1A1*28 (TA)7, *36 (TA)5, *37 (TA)8), and to detect a single-nucleotide mutation of UGT1A1 c.211G>A (rs4148323, UGT1A1*6). A wild-type variation of the gene, a UGT1A1*1 allele, carries 6 TA-repetitions. Homozygous UGT1A1*28 variant is, in Caucasian and Afro- American populations, the most frequent cause of Gilbert syndrome, a benign disorder associated with a mild chronic hyperbilirubinemia. In that case, the glucuronidation activity of UGT1A1 enzyme is reduced to 20-30%. In Asian populations, the main cause of Gilbert syndrome is polymorphism UGT1A1*6, in Afro-American ethnicities, it is the *36 and *37 polymorphism. Genetic variations within the UGT1A1 gene have also been associated with the development of certain drug toxicities. The UGT1A1*28 allele is associated with neutropenia and diarrhea in patients receiving the chemotherapeutic drug irinotecan.
Parameters of the real-time PCR diagnostic kit
- sample concentration 2.5-100 ng/µl
- positive and negative controls included
- multiplex FAM and HEX channel detection
|Cat. No.||Product||No. of reactions||Price|
|3253-025||gb PHARM UGT1A1||25||Unavailable|
|3253-050||gb PHARM UGT1A1||50||Unavailable|
1 CE IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (20 μl volume of each reaction).
Shiping on dry ice from
€ 100 (EU)
For other destinations see indicative list.
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