Detection method: melting analysis
This in vitro diagnostic kit enables DNA genotyping in terms of the presence of 5TA (UGT1A1*36), 6TA (UGT1A1*1), 7TA (UGT1A1*28) and 8TA (UGT1A1*37) polymorphisms in the UGT1A1 gen. The detection is based on the Q-FRET probe and uses the analysis of melting curves for genotyping.
The enzyme UDP-glucuronosyltransferase (UGT) is responsible for the conjugation phase of bilirubin biotransformation. The activity of the enzyme is conditioned by the presence of polymorphisms in the UGT1A1 gene. A wild-type variation of the gene, an UGT1A1*1 allele, carries 6 TA-repetitions. Homozygous UGT1A1*28 variant is, in Caucasian and Afro-American populations, the most frequent cause of Gilbert syndrome, a benign disorder associated with a mild chronic hyperbilirubinemia. In that case, the glucuronidation activity of UGT1A1 enzyme is reduced to 20–30%.
Alleles UGT1A1*36 and UGT1A1*37 are mainly presented in Africans ethnic group. Genetic variations within the UGT1A1 gene have also been associated with the development of certain drug toxicities. The UGT1A1*28 allele is associated with neutropenia and diarrhea in patients receiving the chemotherapeutic drug irinotecan.
The examinations are used to confirm the diagnosis of Gilbert’s syndrome, or before the administration of irinotecan drug and other drugs metabolised by UGT.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3253-025 | gb PHARM UGT1A1 | 25 | Not available |
| 3253-050 | gb PHARM UGT1A1 | 50 | Not available |
1 CE IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (20 μl volume of each reaction).
Shiping on dry ice from
€ 110 (EU)
For other destinations see
indicative list.
Validated for:
CFX Opus 96 (Bio-Rad)
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
QuantStudio 5 (Applied Biosystems)
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
Kit for isolation of DNA from body fluids The DNA extraction kit includes DNA purification step and enables achieving highly pure genomic DNA from samples of whole blood, serum, plasma, buccal swab and urine. Moreover, viral DNA can be efficiently isolated from these types of samples as well. High DNA yields, reproducible results and fast working method are guaranteed.
Your request has been sent.
Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
This product has not been validated for your cycler?
Send us the type of your cycler and we will find a solution for you.
Your request has been sent.
Thank you for your interest in our testing services. We will reply to your e-mail address as soon as possible.
Thank you for your inquiry
Thank you for your inquiry
Your registration has been sent.
Thank you for your inquiry
Price will be calculated based on your exact location within 2 working days.
ENG 
CZE