Detection method: melting analysis
This in vitro diagnostic kit allows detection of variants of 5TA (UGT1A1 * 36), 7TA (UGT1A1 * 28), 8TA (UGT1A1 * 37), and 211G / A SNP UGT1A1 polymorphisms. The detection is based on the Q-FRET probe and uses the analysis of melting curves for multiplex genotyping.
The UGT1A1 gene is a part of a complex locus that encodes several UDP-glucuronosyltransferases, enzymes of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. Mutations in the UGT1A1 gene lead to mulfunctions in conjugation phase of bilirubin biotransformation.
The gb PHARM UGT1A1 diagnostic kit is intended to determine changes in a number of TA-repetitions in a UGT1A1 gene promoter (rs8175347, UGT1A1*28 (TA)7, *36 (TA)5, *37 (TA)8), and to detect a single-nucleotide mutation of UGT1A1 c.211G>A (rs4148323, UGT1A1*6). A wild-type variation of the gene, a UGT1A1*1 allele, carries 6 TA-repetitions. Homozygous UGT1A1*28 variant is, in Caucasian and Afro- American populations, the most frequent cause of Gilbert syndrome, a benign disorder associated with a mild chronic hyperbilirubinemia. In that case, the glucuronidation activity of UGT1A1 enzyme is reduced to 20-30%. In Asian populations, the main cause of Gilbert syndrome is polymorphism UGT1A1*6, in Afro-American ethnicities, it is the *36 and *37 polymorphism. Genetic variations within the UGT1A1 gene have also been associated with the development of certain drug toxicities. The UGT1A1*28 allele is associated with neutropenia and diarrhea in patients receiving the chemotherapeutic drug irinotecan.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3253-025 | gb PHARM UGT1A1 | 25 |
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| 3253-050 | gb PHARM UGT1A1 | 50 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (20 μl volume of each reaction).
Shiping on dry ice from € 95 (EU)
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Validated for:
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
RG 3000 (Corbett Research)
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
gb Elite PCR Master Mix gb Elite PCR Master Mix falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2 and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.
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Dispatch date
Products are dispatched within 5 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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