gb PHARM CYP2C19

gb PHARM CYP2C19

Detection method: allelic discrimination

Kit for detection of CYP2C19*2, CYP2C19*3 and CYP2C19*17 gene mutation. The detection principle is based on a real-time polymerase chain reaction (qPCR) using fluorescent labeled probes (allelic discrimination).

Clinical implications

CYP2C19 is a clinically relevant enzyme involved in the biotransformation of a range of antidepressant, anticoagulant and antimycotic drugs. Side effects of clopidogrel treatment are associated with severe consequences. Clopidogrel is a very effective antiplatelet drug, the effect of which is based on an irreversible inhibition of platelet receptor P2RY12. It is a pro-drug that is activated by the cytochrome P450 complex, predominantly by the CYP2C19.

Slow metabolizers are the most common carriers of the allele *2 and *3 and ultra-fast metabolism is associated with the allele *17 in the Caucasian population. Slow metabolisers do not produce an active form of the drug and they are in risk of non functional treatment. The ultra-fast metabolisers are in danger of bleeding conditions due to reduced platelet activity.

Cat. No. Product No. of reactions Price
3254-025 gb PHARM CYP2C19 25
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3254-050 gb PHARM CYP2C19 50
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1 kit contains reagents to provide 25 or 50 PCR reactions (20 μl volume of each reaction).

Validated for:

ABI 7500/7500 Fast (ABI)

CFX96/96Touch (Bio-Rad)

RG 3000 (Corbett Research)

RG 6000/Q (Corbett Research/Qiagen)

Select further cyclers

Parameters of the diagnostic kit

  • ready-to-use assay
  • sample concentration 2-100 ng/µl
  • positive and negative controls included
  • FAM and HEX channels detection
  • identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
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