Detection method: quantitative real-time PCR, real-time PCR
This kit is intended for detection of the specific section of the POLR2A gene in the human genomic DNA. Detection is based on quantitative real-time polymerase chain reaction (qPCR) using fluorescently labelled probes.
The quantification of human DNA is significant especially in the field of forensic analysis but it could be also exploitable in any other discipline dealing with human DNA analysis. It is essential before sample analysis, when it is important to find out if human DNA is present in the sample and possibly in which amount. The POLR2A gene encodes for the largest subunit of RNA polymerase II, an enzyme responsible for synthesizing messenger RNA in eukaryotes.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3142 | gb GENETIC Human DNA | 100 |
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1 RUO real-time PCR kit contains reagents to provide 100 PCR reactions (25 μl volume of each reaction).
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General Terms and Conditions
gb OneStep RT-qPCR Kit gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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