gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.
gb GENETIC human DNA
Detection method: real-time PCR
This kit is intended for detection of the specific section of the POLR2A gene in the human genomic DNA. Detection is based on quantitative real-time polymerase chain reaction (qPCR) using fluorescently labelled probes.
Clinical implications of the RUO kit
The quantification of human DNA is significant especially in the field of forensic analysis but it could be also exploitable in any other discipline dealing with human DNA analysis. It is essential before sample analysis, when it is important to find out if human DNA is present in the sample and possibly in which amount. The POLR2A gene encodes for the largest subunit of RNA polymerase II, an enzyme responsible for synthesizing messenger RNA in eukaryotes.
Parameters of the real-time PCR diagnostic kit
- ready-to-use assay
- positive and negative controls included
- detection in FAM channel
- internal positive control in HEX channel
|Cat. No.||Product||No. of reactions||Price|
|3142||gb GENETIC human DNA||100||
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1 RUO real-time PCR kit contains reagents to provide 100 PCR reactions (25 μl volume of each reaction).
Shiping on dry ice from
€ 100 (EU)
For other destinations see indicative list.