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gb GENETIC Human DNA

Detection method: quantitative real-time PCR

This kit is intended for detection of the specific section of the POLR2A gene in the human genomic DNA. Detection is based on quantitative real-time polymerase chain reaction (qPCR) using fluorescently labelled probes.

Clinical implications of the RUO kit

The quantification of human DNA is significant especially in the field of forensic analysis but it could be also exploitable in any other discipline dealing with human DNA analysis. It is essential before sample analysis, when it is important to find out if human DNA is present in the sample and possibly in which amount. The POLR2A gene encodes for the largest subunit of RNA polymerase II, an enzyme responsible for synthesizing messenger RNA in eukaryotes.

Parameters of the real-time PCR diagnostic kit

ready-to-use assay
positive and negative controls included
detection in FAM channel
internal positive control in HEX channel

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Cat. No.	Product	No. of reactions	Price

3142

gb GENETIC Human DNA

100

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1 RUO real-time PCR kit contains reagents to provide 100 PCR reactions (25 μl volume of each reaction).

Shiping on dry ice from € 110 (EU)
For other destinations see indicative list.

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General Terms and Conditions

Verified for:

CFX96/96Touch (Bio-Rad)

Select further cyclers

Useful links

gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Alternative dyes for oligonucleotides/probes labeling

Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.