Detection method: one-step real-time PCR
This in vitro diagnostic kit enables the quantitative determination of the copy number ratio of the e1a2 transcript of the BCR-ABL1 fusion gene to the copy number of the ABL1 reference gene transcript.
The BCR-ABL1 fusion gene in the rare MINOR variant occurs in patients with chronic myeoloid leukemia (CML) and Ph+ acute lymphoblastic leukemia (ALL). Patient response to treatment as well as minimal residual disease is monitored by quantification of BCR-ABL1 transcript relative to control gene transcript (ABL1).
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3247-048 | gb ONCO BCR-ABL MINOR / ABL | 48 | Not available |
1 CE IVD one-step real-time PCR kit contains reagents to provide 48 PCR reactions (25 μl volume of each reaction).
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€ 110 (EU)
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indicative list.
General Terms and Conditions
Validated for:
ABI 7500/7500 Fast (ABI)
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
QuantStudio 5 (Applied Biosystems)
QuantStudio 7 (Applied Biosystems)
RG 3000 (Corbett Research)
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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