Detection method: semi-quantitative real-time PCR
The CE IVD real-time PCR kit is intended for semi-quantitative determination of the JAK2 V617F mutation status (c.1849G>T; p.Val617Phe) in exon 14 of the JAK2 gene encoding Janus kinase 2. The detection provides information about the presence of the V617F mutation in the gDNA sample; an indicative estimate of relative mutation load may be provided as supplementary information. The detection principle is based on real-time PCR using fluorescently labeled probes and allele specific primers.
The JAK2 enzyme is involved in the signaling pathway leading to blood cell production. Substitution of the amino acid valine for phenylalanine at position 617 leads to uncontrolled cell proliferation and survival of hematopoietic precursors. Somatic mutation occurs in most patients with diseases such as essential thrombocythemia and chronic idiopathic myelofibrosis. A higher risk of complications (thrombosis, abortion, etc.) is generally expected in carriers of this mutation.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3286-024 | gb ONCO JAK2 (V617F) | 24 |
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| 3286-048 | gb ONCO JAK2 (V617F) | 48 |
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1 CE IVD real-time PCR kit contains reagents to provide 24 or 48 PCR reactions (25 μl volume of each reaction).
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General Terms and Conditions
Validated for:
CFX Opus 96 (Bio-Rad)
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
QuantStudio 5 (Applied Biosystems)
Kit for isolation of DNA from body fluids The DNA extraction kit includes DNA purification step and enables achieving highly pure genomic DNA from samples of whole blood, serum, plasma, buccal swab and urine. Moreover, viral DNA can be efficiently isolated from these types of samples as well. High DNA yields, reproducible results and fast working method are guaranteed.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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