Detection method: quantitative real-time PCR
This kit is used for detection and quantification of somatic mutation V617F JAK2 gene in human genomic DNA. The detection principle is based on real-time PCR using fluorescently labeled probes and allele specific primers.
The JAK2 enzyme is involved in the signaling pathway leading to blood cell production. Substitution of the amino acid valine for phenylalanine at position 617 leads to uncontrolled cell proliferation and survival of hematopoietic precursors. Somatic mutation occurs in most patients with with diseases such as essential thrombocythemia and chronic idiopathic myelofibrosis.
A higher risk of complications (thrombosis, abortion, etc.) is generally expected in carriers of this mutation.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3242-024 | gb ONCO JAK2 (V617F) | 24 |
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| 3242-048 | gb ONCO JAK2 (V617F) | 48 |
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1 real-time PCR kit contains reagents to provide 24 or 48 PCR reactions (25 μl volume of each reaction).
Selling, offering, storage and usage of the product in some countries may infringe valid patent rights under patent EP 1692281 and its equivalents.
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General Terms and Conditions
Verified for:
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
RG 6000/Q (Corbett Research/Qiagen)
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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The avermectins compose a class of polycyclic lactones that are used to treat parasitic worms and insect pests. They are naturally occurring compounds generated in a fermentation process by a soil actinomycete Streptomyces avermitilis.
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Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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