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gb ONCO JAK2 (V617F)

Detection method: quantitative real-time PCR

This kit is used for detection and quantification of somatic mutation V617F JAK2 gene in human genomic DNA. The detection principle is based on real-time PCR using fluorescently labeled probes and allele specific primers.

Clinical implications of the kit

The JAK2 enzyme is involved in the signaling pathway leading to blood cell production. Substitution of the amino acid valine for phenylalanine at position 617 leads to uncontrolled cell proliferation and survival of hematopoietic precursors. Somatic mutation occurs in most patients with with diseases such as essential thrombocythemia and chronic idiopathic myelofibrosis.
A higher risk of complications (thrombosis, abortion, etc.) is generally expected in carriers of this mutation.

Parameters of the real-time PCR diagnostic kit

ready-to-use assay
LOD 0.26% mutated JAK2 on WT background at 100,000 copies in reaction
sample concentration 4-80 ng/µl
positive and negative controls included
FAM channel detection
identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits

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Cat. No.	Product	No. of reactions	Price

3242-024	gb ONCO JAK2 (V617F)	24	Add to Quote Add to Quote
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3242-048

gb ONCO JAK2 (V617F)

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1 real-time PCR kit contains reagents to provide 24 or 48 PCR reactions (25 μl volume of each reaction).

Selling, offering, storage and usage of the product in some countries may infringe valid patent rights under patent EP 1692281 and its equivalents.

Shiping on dry ice from € 115 (EU)
For other destinations see indicative list.

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General Terms and Conditions

Verified for:

CFX96/96Touch (Bio-Rad)

Light Cycler 480/Cobas z480 (Roche Diagnostics)

QuantStudio 5 (Applied Biosystems)

RG 6000/Q (Corbett Research/Qiagen)

Select further cyclers

Useful links

gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Alternative dyes for oligonucleotides/probes labeling

Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.