Detection method: quantitative real-time PCR
This kit is used for detection and quantification of somatic mutation V617F JAK2 gene in human genomic DNA. The detection principle is based on real-time PCR using fluorescently labeled probes and allele specific primers.
The JAK2 enzyme is involved in the signaling pathway leading to blood cell production. Substitution of the amino acid valine for phenylalanine at position 617 leads to uncontrolled cell proliferation and survival of hematopoietic precursors. Somatic mutation occurs in most patients with with diseases such as essential thrombocythemia and chronic idiopathic myelofibrosis.
A higher risk of complications (thrombosis, abortion, etc.) is generally expected in carriers of this mutation.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3242-024 | gb ONCO JAK2 (V617F) | 24 |
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| 3242-048 | gb ONCO JAK2 (V617F) | 48 |
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1 real-time PCR kit contains reagents to provide 24 or 48 PCR reactions (25 μl volume of each reaction).
Selling, offering, storage and usage of the product in some countries may infringe valid patent rights under patent EP 1692281 and its equivalents.
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General Terms and Conditions
Verified for:
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
QuantStudio 5 (Applied Biosystems)
RG 6000/Q (Corbett Research/Qiagen)
Dual-labeled hydrolysis probes The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
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Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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