Categories

gb ONCO JAK2 (V617F) gb ONCO JAK2 (V617F)

gb ONCO JAK2 (V617F)

Detection method: quantitative real-time PCR

This in vitro diagnostic kit is used for detection and quantification of somatic mutation V617F JAK2 gene in human genomic DNA. The detection principle is based on real-time PCR using fluorescently labeled probes and allele specific primers.

Clinical implications of the IVD kit

The JAK2 enzyme is involved in the signaling pathway leading to blood cell production. Substitution of the amino acid valine for phenylalanine at position 617 leads to uncontrolled cell proliferation and survival of hematopoietic precursors. Somatic mutation occurs in most patients with with diseases such as essential thrombocythemia and chronic idiopathic myelofibrosis.
A higher risk of complications (thrombosis, abortion, etc.) is generally expected in carriers of this mutation.

Parameters of the real-time PCR diagnostic kit

  • ready-to-use assay
  • LOD 0.1% mutated JAK2 on WT background at 100,000 copies in reaction
  • sample concentration 4-80 ng/µl
  • positive and negative controls included
  • FAM channel detection
  • identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
Cat. No. Product No. of reactions Price
3242-025 gb ONCO JAK2 (V617F) 25
Add to Quote
Add to Quote
3242-050 gb ONCO JAK2 (V617F) 50
Add to Quote
Add to Quote

1 IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (25 μl volume of each reaction).

Selling, offering, storage and usage of the product in some countries may infringe valid patent rights under patent EP 1692281 and its equivalents.

Shiping on dry ice from € 95 (EU)
For other destinations see indicative list.

Verified for:

CFX96/96Touch (Bio-Rad)

Light Cycler 480/Cobas z480 (Roche Diagnostics)

Select further cyclers

Useful links
Recommended products
New
gb OneStep RT-qPCR Kit

gb OneStep RT-qPCR Kit gb OneStep RT-qPCR Kit

gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.

More
Recommended articles
CRO case studies

Yeast Production Strain Cell Line Authentication by RFLP analysis

Yeast Production Strain Cell Line Authentication by RFLP analysis

Customer need Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of

More