The CE IVD real-time PCR kit is intended for a qualitative determination of a type of the BCR-ABL1 fusion gene alteration. Apart from commonly occurring fusions the kit detects rare alterations as well. Detection method based on one-step RT-qPCR ensures quick, less laborious and, above all, semiquantitative determination of the fusion type, while sophisticated system of controls ensures validity of the result and its adequate interpretation.
The gb ONCO BCR-ABL DETECT diagnostic kit enables the identification or detection of the BCR-ABL1 fusion gene breakpoints variants in patients with a hemato-oncological disease.
The aim of BCR-ABL1 breakpoint variant determination is the confirmation of a diagnosis, the determination of a prognosis and the setting up of a treatment plan for patients with e.g. chronic myeloid leukaemia (CML), acute lymphoblastic leukaemia (ALL) and for patients after bone marrow transplantation.
The test is based on one-step RT-qPCR using fluorescently labeled probes. The kit enables detection of the following BCR-ABL1 fusion variants:
| FAM/SYBR | HEX/JOE/VIC | ROX | Cy5 | |
| Assay BCR-ABL DETECT 1 | e14a2 | e13a2 | reference GUSB | e14a3 e13a3 |
| Assay BCR-ABL DETECT 2 | e1a2 e1a3 |
e19a2 e19a3 |
e6a2 | reference GUSB |
The validity of the analysis of the samples is evaluated according to the level of the reference GUSB gene transcript and the homogeneity of its analysis by both of the assays.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3246-048 | gb ONCO BCR-ABL DETECT | 48 |
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1 CE IVD one-step real-time PCR kit contains reagents to provide 48 PCR reactions (25 μl volume of each reaction).
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€ 110 (EU)
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indicative list.
General Terms and Conditions
Validated for:
CFX96/96Touch (Bio-Rad)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
Dual-labeled hydrolysis probes The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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