gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.
gb ONCO BCR-ABL DETECT
The CE IVD real-time PCR kit is intended for a qualitative determination of a type of the BCR-ABL1 fusion gene alteration. Apart from commonly occurring fusions the kit detects rare alterations as well. Detection method based on one-step RT-qPCR ensures quick, less laborious and, above all, semiquantitative determination of the fusion type, while sophisticated system of controls ensures validity of the result and its adequate interpretation.
The gb ONCO BCR-ABL DETECT diagnostic kit enables the identification or detection of the BCR-ABL1 fusion gene breakpoints variants in patients with a hemato-oncological disease.
The aim of BCR-ABL1 breakpoint variant determination is the confirmation of a diagnosis, the determination of a prognosis and the setting up of a treatment plan for patients with e.g. chronic myeloid leukaemia (CML), acute lymphoblastic leukaemia (ALL) and for patients after bone marrow transplantation.
Principle of detection
The test is based on one-step RT-qPCR using fluorescently labeled probes. The kit enables detection of the following BCR-ABL1 fusion variants:
|Assay BCR-ABL DETECT 1||e14a2||e13a2||reference GUSB||e14a3
|Assay BCR-ABL DETECT 2||e1a2
The validity of the analysis of the samples is evaluated according to the level of the reference GUSB gene transcript and the homogeneity of its analysis by both of the assays.
Parametres of the diagnostic kit
- in vitro diagnostics
- CE IVD marked
- detection in the following channels: FAM, HEX, ROX, and Cy5
|Cat. No.||Product||No. of reactions||Price|
|3246-048||gb ONCO BCR-ABL DETECT||48||
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1 CE IVD one-step real-time PCR kit contains reagents to provide 48 PCR reactions (25 μl volume of each reaction).
Shiping on dry ice from
€ 100 (EU)
For other destinations see indicative list.
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