Our modified oligonucleotides cover end modifications at the 3′ and 5′ ends as well as internal positions.
We offer base analogs, spacers, fluorophores, quenchers, backbone changes and functional groups for applications such as antisense, sequencing, duplex stabilization, structural studies or click-chemistry conjugations.
All modifications are delivered with high purity and can be combined on request (where chemistry allows).
Popular modifications & use cases
Looking for labeled probes? See
RT-PCR dual-labeled probes
or browse all oligonucleotides.
HEX Hexachlorofluorescein is commonly used in multiplex reactions together with FAM and TET. Hexachlorofluorescein may be attached to the 5' end of the oligonucleotide.
Hexynyl It is used for conjugations based on the click chemistry principle. Hexynyl may be attached to the 5' end of the oligonucleotide.
Cholesterol Adding cholesterol increases oligonucleotide lipophility. Furthermore, cholesterol improves the permeability of oligonucleotide chains through cellular membranes in antisense oligonucleotides (phosphorothioates or other derivatives). Cholesterol may be attached either to the 5' end or the 3' end of the oligonucleotide.
JOE 6-karboxy-4', 5'-dichlor-2', 7'-dimethoxyfluorescein is commonly used in multiplex reactions together with FAM. JOE may be attached to the 5' end of the oligonucleotide.
Inosin 2'deoxyinosin is a "universal" base (it has the ability to bind to each of the 4 standard bases). It can be located anywhere in the oligonucleotide chain.
ROX ROX (6-Carboxy-X-Rhodamine) is a red fluorophore. It can be linked to the 3 'end of the chain, or by using the NHS ester at 5'. In case of binding at the 5 'end, the amino linker is first attached to the oligonucleotide through which the post-synthetic binding of ROX then proceeds.
Spacer 18 May be attached anywhere within the oligonucleotide sequence except for the 3' end. It is the longest spacer available within a single modification.
Spacer C3 May be attached anywhere within the oligonucleotide sequence. Repeated spacer attachment is possible, forming a long hydrophobic arm. When bound to the 3' end it serves as a blocker of 3' polymerase extension.
Spacer C12 May be attached anywhere within the oligonucleotide sequence except for the 3' end. Repeated spacer attachment is possible.
Your request has been sent.
Your cycler has not been included in the list?
Send us the type of your cycler and we will find a solution for you.
Your request has been sent.
Thank you for your inquiry
Thank you for your inquiry
Your registration has been sent.
Thank you for your inquiry
ENG 
CZE 
